Tubing-Line and Weight
Upon completion of lower depth sampling, the sampling
line and support line are retrieved by slowly pulling on the Kevlar
line (Dirty-hands person) while the clean-hands person coils the
tubing into the storage bag. The sample line tubing should be
un-coupled from the pumptubing before retrieval so that the river
water drains out. Untie the support line and seal in storage box.
The weight and tubing must be flushed with dilute acid to prevent
cross-contamination and to prevent contaminant build-up. A dilute
acid solution is supplied in a 1 gallon PE bottle. Before inserting
the tubing weight into the acid bottle, wipe the top outer surfaces
of the weight with a clean-room wiper. Insert the tubing weight
into the acid bottle, connect the free end of the sampling line
to the pump-head tubing, and flush at moderate-high pump speed.
Pump until all of the acid solution has flushed through the tubing,
and then continue pumping until a majority of the tubing has been
pumped dry (you may have to lift the tubing weight out of the
acid jar to ensure that the tubing pumps dry). Remove the weight
from the acid bottle, place in a new plastic bag, and wipe the
top outer surfaces with a new clean room wiper. Recoil the tubing,
tie with an arm length glove, and place in plastic bag. Store
in dedicated storage container. Tubing will be periodically resupplied
from the Water Chemistry Lab.
Calex filter is discarded. Rinse PCL and TTAF with
MQ, double-bag using clean techniques, and place in tubing storage
container. (The TTAF fittings should be periodically returned
to Madison along with samples for more rigorous cleaning). Canopy
bag is discarded. Platform is rinsed with MQ and wiped with clean-room
wipers. Canopy frame and platform are bagged and placed in rubbermaid
Bagged in two large PE bags. Fiberglass cleat adaptor
is bagged and stored in supplies container.
Anchors and Anchor Line
Any sediment on anchors or line is washed off in
the river before bringing into the boat. When anchor is clean,
remove from water and place directly into a plastic bag.
The inside surfaces of the boat must be rinsed with
water after sampling is completed. If simple flushing with water
is not sufficient to remove grime, then use the supplied brush
to loosen dirt.